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분야
pptxAnimal : New Zealand white rabbits coronary arterial (4-5mm in length)
Endothelium of the arterial ring was remove
0Ca2+-1mM EGTA sol. : KH sol. and addition of 1mM EGTA
2. Single-cell isolation and Ca2+ imaging
Stips loaded with 1uM fura-2/AM in Tyrode’s solution for 30min at room temperature
[R(F340/F380)], 510nm CCD camera
Dissociation constant of fluorescence indicator for Ca2+
3. Permeabilization with ß-escin and force recording
Permeabilized strips : 200um wide and 2-3mm long
Permeabilization : Incubated at room temperature in ß-escin(30uM) in relaxing sol. (4.5mM MgATP, 1mM EGTA etc.) for 25min
Our results suggest that constriction of arteries in response to the bioactive lipid S1P or SPC occurs by distinct signalling pathways. Activation of the RhoA/RhoA-associated kinase pathway and subsequent phosphorylation of MYPT1 play a key role in SPC-induced coronary contraction, whereas elevation of [Ca2+]i is crucial for S1P-induced coronary constriction.
PPT에 발표 스크립트도 포함되어 있습니다.
논문읽고 발표수업 하실 때 참고하시면 많은 도움 되실겁니다.
