소개글
Cell line 레포트에 대한 자료입니다.
목차
(1). Introduction
(2). Animal / Human cell
(3). Plant / Insect cell
(4). Stem cell
(5). Experimental handling of cell line culture
(6). A proteomic study of cMyc improvement of CHO culture
본문내용
3. 암화에 의한 세포주 확립
동물세포를 이용한 연구 발전에 기여
4. Sato등에 의해 무혈청 배양법 확립
1960. 무혈청 배양 시도
1963-5(Ham) 혈청 공급 없이
CHO cell cloning
1970(Sato) 혈청 대신 호르몬
첨가 cell line 무혈청 배양
성공
Human embryonal kidney, SV40 and large T antigen transformed
produce therapeutic proteins and viruses for gene therapy
very easy to grow
transfect very readily
Contains the
SV40 Large T-antigen
Design
A callus cell culture is usually sustained on gel media, in much the same manner as bacteria are grown. Sufficient media consist of agar and the usual mix of macronutrients and micronutrients for the given cell type. For plant cells, enrichment with nitrogen, phosphorus, and potassium is especially important. Water is provided as a constituent part of the gel media.
Uses
A plant cell callus consists of somatic undifferentiated cells from an adult subject plant.
A callus is not necessarily genetically homogeneous because a callus is often made from structural tissue, not individual cells. Nevertheless, callus cells are often considered similar enough for standard scientific analysis to be performed as if on a single subject. For example, an experiment may have half a callus undergo a treatment as the experimental group, while the other half undergoes a similar but non-active treatment as the control group.
Plant cell calluses may be made to differentiate into the specialized tissues of a whole plant, with the addition of a number of hormones or enzymes. This is an ability known as totipotency.
A callus is often the target of a gene gun for specific DNA insertion experiments.
Callus tissue is of particular use in micropropagation where it can be used to grow genetically identical copies of plants with desirable characteristics.
참고문헌
References
A proteomic study of cMyc improvement of CHO culture, Kuystermans et al. BMC Biotechnology 2010, 10:25
The cell, Cooper&Geoffrey, Sinauer AssociatesInc, 2009(5th)
Introduction to Proteomis, Kraj et al. Wiley&SonsInc, 2008
Websites
http://www.genego.com/maps