분야
pptx2. Introduction
3. Methods & Material
4. Results
5. Discussion & conclusion
6. Reference
To understand Plant stress responses
To find glucose responses on plant germination and growth.
To understand plant glucose and hormonal interplay
2. Introduction
▶ Plant stress
External conditions that adversely affect growth,
development, or productivity
Stresses trigger a wide range of plant responses:
altered gene expression
cellular metabolism
changes in growth rates and crop yields
▶ Plant stress factors
Biotic - imposed by other organisms
Abiotic - arising from an excess or deficit in the
physical or chemical environment
-> Plant responses to abiotic factors :
arising from an excess or deficit in the
physical or chemical environment
-> Excess exogenous Glucose is also a environmental cue!
▶ Glucose treatment : 3%, 4%, 5% Glc medium
** 전부 clean bench에서 무균작업으로 수행할 것
준비한 종자를 적정량 1.5ml tube에 담고
70% Ethanol 200ul씩 3회 washing
2% NaOCl 200ul 10분 treatment
3차증류수 200ul 5회 washing
Washing 후 다시 3차 증류수 200ul을 넣은 뒤 pipetting으로 종자를 배지 위에 물기가 없도록 올려놓는다.
2주간 관찰, 발아율, 뿌리길이, cotyledon 활착 여부, 엽색의 변화 등을 관찰
Sheen, J., Zhou, L., & Jang, J. C. (1999). Sugars as signaling molecules. Current opinion in plant biology, 2(5), 410-418.
Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and environmental safety, 60(3), 324-349.
Arenas-Huertero, F., Arroyo, A., Zhou, L., Sheen, J., & León, P. (2000). Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes & Development, 14(16), 2085-2096.
Li, Y., Lee, K. K., Walsh, S., Smith, C., Hadingham, S., Sorefan, K., ... & Bevan, M. W. (2006). Establishing glucose-and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine. Genome Research, 16(3), 414-427.
Xiao, W., Sheen, J., & Jang, J. C. (2000). The role of hexokinase in plant sugar signal transduction and growth and development. Plant molecular biology, 44(4), 451-461.
http://en.wikipedia.org/wiki/Abscisic_acid
http://en.wikipedia.org/wiki/Ethylene#Ethylene_as_a_plant_hormone
