|A simple and reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the analysis of the flowers of Chrysanthemum morifolium (CM). Luteolin-7-Oglucoside (LU7G) was chosen as a target analyte considering its content, availability, and ease of analysis. Chromatographic separation of LU7G was achieved using a Phenomenex Gemini C18 column (250 × 4.6 mm, 5 μm) run with a mobile phase consisting of 0.5 % acetic acid in water and 0.5 % acetic acid in acetonitrile at a flow rate of 1.0 mL min−1. The detection wavelength and column temperature were set at 350 nm and 40 ℃, respectively. Method validation was performed according to the AOAC guidelines and the method was specific, linear (R² = 0.9991 for 50–300 μg mL−1), precise (≤ 3.91 % RSD), and accurate (100.1–105.7 %). The limits of detection and quantification were 3.62 and 10.96 μg mL−1, respectively. The established method was successfully applied to determine the contents of LU7G in various batches of bulk CM extracts and labscale CM extract. The developed method is a readily applicable method for the quality assessment of CM and its related products.