Six soybean varieties including recommended variety, local and imparted soybean Variety were investigated the effect on the growth of soybean sprouts under the various temperatures of culture and water supply, and to develop the culture methods for the production of clean soybean sprouts. The highest internal temperature of cultural vessels was recorded at 5th day during sprouts culture and the temperature was increased up to 29.5℃ as the water spraying time was elapsed. Germination speed, whole length, hypocotyl length, and yield of soybean sprouts wire increased at the high culture temperature condition(25 and 30℃), and the sprouts` rotting was started at 5th day during sprout culture but not observed at 17℃ and 20℃ culture condition. The yield and characteristics of soybean sprouts was more affected by the culture temperature than the temperature of supplied water. The 20℃ was recommended as an adequate cultural temperature and supplying water, respectively, for the soybean sprouts culture. However the hypocatyl/whole length ratio of soybean sprouts produced under the recommended temperature condition was below than 50%.