참붕어, Pseudorasbora parva의 인두흡충, Clinostomum complanatum 피낭유충 감염증
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Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were 4.02±1.80 ㎜(1.66-5.81) in length and 1.27±0.27 ㎜(1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring 0.18±0.05 ㎜ (0.11-0.24) in length and 0.23±0.07 ㎜(0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured 0.47±0.08 ㎜(0.35-0.53) in length and 0.55±0.09 ㎜ (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.
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