Purpose: This study was conducted in order to investigate the relationship between the level of emotional labor and job stress for public health nurses. Methods: The survey was conducted from Mar. to Oct. 2010 for collection of data from public health nurses (N=195). Descriptive statistics, ANOVA, t-test, Tukey HSD, and Pearson`s correlation coefficient were used in performance of data analysis. Results: Results of this study indicate that as the nurses grow older, they receive a larger salary and their emotional labor is reduced. The results of this study also demonstrate that the emotional labor is highest when their total nursing job work duration and public health nurse work duration are between six and 10 years. There is a significant difference in job stress of the two age groups between occupational roles and personal strain. This means that job stress is highest in nurses who are in their thirties and low fifties. Emotional labor showed positive correlation with occupational roles and personal strain of job stress, but showed negative correlation with personal resources. Conclusion: According to the results of this study, we suggest development of interventions to reduce emotional labor and job stress, taking into account the characteristics of age.