Transient receptor potential vanilloid type 1 (TRPV-1) plays a crucial role in pain perception and development of hyperalgesia, and its expression is up-regulated in patients with irritable bowel syndrome (IBS). Although several non-synonymous single nucleotide polymorphisms (SNPs) in human TRPV-1 gene have been identified, there is no information about their pathobiological significance in IBS. The aim of this study was to investigate potential association between SPNs of the TRPV-1 gene and patients with IBS. Methods: We chose to focus on three SNPs in human TRPV-1 coding region (rs222749, rs9894618 and rs222747) in 80 healthy control and 104 IBS patients. We developed the high resolutional melting (HRM) method to determine the genotyping of the rs222747 and rs9894618 and the genotyping of the rs222749 was also determined by direct sequencing method. Results: The CG genotype of the rs222747 was 58.8% (47/80) in controls and 46.2% (48/104) in IBS patients (OR 0.581, 95% CI 0.277-1.217, p=0.150). The GG genotype of the rs222747was 15.0% (12/80) in controls and 20.2% (21/104) in IBS patients (OR 1.135, 95% CI 0.428-3.009, p=0.798).The CT genotype of the rs222749 was 31.3% (25/80) in control group and 31.7% (33/104) in IBS patients (OR 1.145, 95% CI 0.568-2.308, p=0.705). There was no significant difference in allele frequency of the SNPs of TRPV-1 gene between healthy control and IBS patients. Conclusion: These results suggest that the SNPs of TRPV-1 gene may not be associated with IBS in Korean populations. Further studies with large cases are needed to validate the results of the present study.