|Objective: brachial-ankle pulse wave velocity (baPWV) is widely used as a marker of arterial stiffness and increased arterial stiffness is an important risk factor for cardiovascular disease. Although diabetes mellitus (DM) and hypertension (HTN) are major risk factor for atherosclerosis, the impact of DM and HTN on pulse wave velocity (PWV) has not been well defined. Material and methods: DM with hypertensive patients (Group I, n=467, 232 men, 63±11 years) and hypertensive patients (Group II, n=348, 196men, 59±13 years) who undergone testing a baPWV were included. The measurement of the baPWV were performed in a quiet controlled room (22±1 °C) in the supine position with using an automated device (VP-2000, Colin, Co. Ltd, Komaki, Japan). Results: baPWV is significantly higher in Group I than Group II (1714±360 cm/sec vs. 1620±306 cm/sec, p<0.001). Multivariate linear regression analysis showed significant association of Ln(baPWV) with age (beta=0.008, p=0.001), mean arterial pressure (beta=0.004, p=0.001), heart rate (beta=0.004, p=0.001) and DM &HTN (beta=0.025, p<0.05), R2=0.036 in all study group. Conclusion: baPWV is higher in DM with hypertensive patients than hypertensive patients and this study suggest that DM and HTN may play a significant role in the arterial stiffness.