Citrullinated Fibrinogen Peptide, FibαR84Cit, Is Not a T Cell Epitope in Rheumatoid Arthritis
의약학 > 내과학
( Hyo Park ) , ( Seung Cheol Shim ) , ( Mi Kyung Lim ) , ( Dong Hyuk Sheen )
대한내과학회 추계학술발표논문집 2011년, 제2011권 제1호, 329(총1페이지)
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    Purpose: Immunity to citrullinated protein is highly specific and pathogenic for RA.The purpose of this study is to investigate whether CD4 Tcell responses specific to peptide within the citrullinated fibrinogen are present in patients with RA. Method: CD4 Tcells from peripheral blood mononuclear cells of 34 RA patients and control subjects were stimulated in the presence of citrullinated fibrinogen peptide(FibαR84Cit) that was identified as a DRB1*0401 restricted Tcell epitope in HLA-DR4 transgenic mice or non-citrullinated fibrinogen peptide. Citrullinated and non-citrullinated fibrinogen peptides were chemically synthesized showing over90% purity.HLA-DRB1 genotyping was performed by the PCR-SSPmethod.The level of anti-CCP antibodies was measured using DIASTAT anti-CCP kit. Result: T cell responses were assessed in various groups of subjects according to the presence of ACPA, or specific HLA-DRB1 allele.Proliferative response to the FibαR84Cit was not detected in CD4 T cells from all RA patients irrelevant to patients``specific phenotype(1).Further, the levels of IFN-γ or IL-17A have not been increased in CD4 Tcells stimulated with the FibαR84Cit from patients with RA(2)although they responded to recall antigen,a mixture of tetanus toxoid, purified protein derivative from Mycobacterium tuberculosis, and Candida albicans. Conclusion: There is no evidence of CD4 T cell mediated immune responses to the citrullinated fibrinogen peptide, FibαR84Cit,in the patients with RA indicating anti-citrulline immunity in RA is unlikely to be mediated by FibαR84Cit.
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