재조합 Lactobacillus paracasei로부터 Bacillus subtilis의 Mannanase 생산과 효소특성
분야
자연과학 > 생물
저자
윤기홍 ( Ki Hong Yoon )
발행기관
한국미생물생명공학회(구 한국산업미생물학회)
간행물정보
한국미생물·생명공학회지 2012년, 제40권 제3호, 186~189쪽(총4쪽)
파일형식
05212033.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    A gene coding for mannanase (manA) from Bacillus subtilis was introduced into a shuttle vector pGK12 between Escherichia coli, B. subtilis and Lactobacillus paracasei. As a result of transferring the resultant plas-mid, designated pGK12M3, into three different strains, the manA gene was found to be expressed in L. paracasei as well as in B. subtilis and E. coli. In a 4 L fermentor culture, the production of mannanase by recombinant L. paracasei (pGK12M3) reached a maximum level of 5.4 units/ml in an MRS medium with a fixed pH 6.5. Based on the zymogram of mannanase, it is assumed that mannanase produced by recombinant L. paracasei is not maintained stably with proteolytic degradation. The optimal temperature and thermostability of mannanase produced by recombinant L. paracasei were also found to be different from those of enzymes produced by B. subtilis.
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