Optimization of Extraction Parameters for Keratinase Recoveryfrom Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S
농학 > 농화학
( Hoda Mohamed ) , ( Abdel Halim Shata ) , ( Mohamed Abdel ) , ( Fattah Farid )
한국응용생명화학회(구 한국농화학회)
Journal of Applied Biological Chemistry 2012년, 제55권 제3호, 149~156페이지(총8페이지)
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    The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery ofkeratinase from solid-state fermentation (SSF) of chicken featherby a local Streptomyces sp. NRC 13S were investigated in orderto establish the experimental conditions for keratinase yield.Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevantvariables on keratinase recovery. The factors investigated weresolid/liquid ratio (1:1.66-1:6.66g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The resultsshowed that the maximum recovery of keratinase (6933.3 U/gfs)was obtained using 0.5 (v/v) glycerol as extracting solvent, in asolid /liquid ratio of 1:5 and three extraction cycles.
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