The aim of this research was to investigate the industrialapplication of Sedum sarmentosum. Antibacterial activities of then-hexane, methylene chloride (MC), ethyl acetate, and n-butanolfractions of S. sarmentosum were tested against Escherichia coli,Staphylococcus aureus, and Helicobacter pylori. The MC fractionshowed the strongest antibacterial activity against E. coli, with aninhibition zone greater than 13mm in disc assays. At 100ug/mL all fractions scavenged more than 50% of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (·OH). In particular,the MC fraction showed the strongest scavenging activity against DPPH and ·OH. In addition, we found that treatment with the MC fraction inhibited the growth of H. pylori and gastric adenocarcinomacells. The present results suggest that the MC fraction of S sarmentosum would play the promising protective role againstpathogenic bacteria and free radicals.