Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells
자연과학 > 화학
( Rui Dong Li ) , ( Zhong Liang Deng ) , ( Ning Hu ) , ( Xi Liang ) , ( Bo Liu ) , ( Jin Yong Luo ) , ( Liang Chen ) , ( Liang Jun Yin ) , ( Xiao Ji Luo ) , ( Wei Shui ) , ( Tong Chuan He ) , ( Wei Huang )
생화학분자생물학회(구 한국생화학분자생물학회)
BMB Reports 2012년, 제45권 제9호, 509~514페이지(총6페이지)
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    We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with TGFβ1 during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rhTGFβ1 synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of TGFβ1 inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that TGFβ1 inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in G0/G1 phase. Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells. [BMB Reports 2012; 45(9): 509-514]
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