Objectives: The purpose of this study was to evaluate the dental erosion inhibitory effect of hangover beverage containing calcium and fluoride. Methods: Risk factors of dental erosion in the varying concentrations of fluoride, Ca, P, pH, and the buffer capacity were measured in six groups of mixture: distilled water, Morning care, Morning care adding 3% calcium, Morning care adding 5% calcium, Morning care adding 4 ppm F, Morning care adding 3% calcium and 4 ppm F. Seventy two specimens were prepared for the microhardness tests and divided randomly into 6 groups (n=12). Each group was exposed to the six groups of the mixture for 1, 3, 5, 10, 15, 30 min. Surface microhardness was measured before and after the treatment, and the surface was observed by SEM after the treatment only. Results: After 30 minutes of treatment, the surface microhardness changes were significantly different among the six groups: Distilled water (0.11±1.98 ΔVHN), Morning care (100.49±9.66 ΔVHN), Morning care+3% calcium (17.07±8.45 ΔVHN), Morning care+5% calcium (10.35±7.61 ΔVHN), Morning care+4 ppm F (93.96±15.13 ΔVHN), Morning care+3% calcium+4 ppm F (14.21±7.97 ΔVHN) (P＜0.01). The three groups, including those treated with the mixtures of Morning care+3% calcium, Morning care+5% calcium, and Morning care+3% calcium+4 ppm F, showed no statistically significant difference on the surface microhardness change and inhibition effect on enamel erosion (P＞0.05) when compared with the distilled water group. Conclusions: It is suggested that modification of the Morning care with 3% calcium could be useful for a significant protective potential with respect to dental erosion.