Purpose: The purpose of this study was to compare and quantify the expression of macrophage colony-stimulationg factor (M-CSF), receptor activator of nuclear factor kappa-B (RANK) and osteoprotegerin (OPG) that the related to bone resorption in the gingival tissues of patients with type 2 diabetes mellitus (MD) and healthy adults with chronic periodontitis. Materials and Methods: Depending on the patient`s systemic condition and clinical criteria of gingival, each gingival sample was divided into three groups. Group 1 (n=12) is clinically healthy gingival obtained from systemically healthy 12 patients. Group 2 (n=12) is inflamed gingival from patients with chronic periodontitis. Group 3 (n=12) is inflamed gingival from patients with chronic periodontitis associated with type 2DM. Tissue samples were prepared and analyzed by Western blotting. The relative quantifications of M-CSF,RANK and OPG were performed with a densitometer. Results: The expression levels of M-CSF significantly increased in group 3 as compared to group 1 (P<0.05). The expression levels of RANK were significantly increased in group 3as compared to group 1 and 2. And In the group 2, the levels of RANK were significantly increased as compared to group 1 (P<0.05). The expression levels of OPG were significantly increased in the group 3 as compared to group 1 and 2 (P<0.05). Conclusion: In conclusion, It can be assumed that M-CSF, RANK and OPG may be involved in the progression of periodontal inflammation associated to type 2 DM. This study demonstrated that the expression levels of RANK and OPG might be inflammatory markers in periodontal inflamed tissue.