Purpose The purpose of this study was to identify education, attitudes and beliefs, interest and perceived role, self-efficacy and barriers to physical therapists` implementation of evidence-based practice for people with stroke. Methods The participants were 120 physical therapists providing services to people with stroke in Gwang-ju. The questionnaire contained items to evaluate practitioner and organizational characteristics and perceptions of research considered to influence evidence-based practice. The prevalence of practitioner and organizational factors was estimated using percentages. Logistic regression was used to examine relationships between sociodemographic and practice characteristics and each practitioner factor. Results 40% of respondents had learned the foundations of EBP in their academic preparation or received training in searching or appraising research literature. 88.3% agreed that research findings are useful. Self-efficacy ratings were 45%. Conclusion Lack of education, negative perceptions about research and physical therapists` role in evidencebased practice, and low self-efficacy to perform EBP activities represent barriers to implementating EBP for people with stroke that can be addressed through continuing education.