Purpose: This study was conducted to develop a measurement system for determining photosynthetic photon flux (PPF) distribution and illumination efficiency of LED lamps. Methods: The system was composed of a linear moving sensor part (LMSP), a rotating part to turn the LMSP, a body assembly to support the rotating part, and a motor controller. The average PPF of the LED lamp with natural cooling and water cooling was evaluated using the measurement system. Results: The PPF of LED lamp with water cooling was 3.1-31.7% greater than that with natural cooling. Based on the measured value, PPF on the horizontal surface was predicted. Illumination efficiency of the LED lamp was slightly increased with water cooling by 3.4%, compared with natural cooling. A simulation program using MATLAB was developed to analyze the effects of the vertical distance from lighting sources to growing bed, lamp spacing, and number of LED lamps, on the PPF distribution on the horizontal surface. The uniformity of the PPF distribution of the LED lamps was fairly improved with 15 cm spacing, as compared to the 5 cm spacing. By simulation, PPF of 217.0±27.9umol m-2 s-1 was obtained at the vertical distance of 40 cm from six LED lamps with 12 cm spacing. This simulated PPF was compared to the measured one of 225.9±25.6umol m-2 s-1. After continuous lighting of 346 days, the relative PPF of LED lamps with water cooling and natural cooling was decreased by 6.6% and 22.8%, respectively, Conclusions: From these results, it was concluded that the measurement system developed in this study was useful for determining PPF and illumination efficiency of artificial lighting sources including LED lamp.