Molecular cloning and characterization of a soybean GmMBY184 induced by abiotic stresses
분야
농학 > 생물공학
저자
( Jai Heon Lee ) , ( Eun Sook Chung ) , ( Koung Mee Kim )
발행기관
한국식물생명공학회(구 한국식물조직배양학회)
간행물정보
Journal of Plant Biotechnology(구 식물생명공학회지) 2012년, 제39권 제3호, 175~181쪽(총7쪽)
파일형식
05807198.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.
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