Physicochemical, dye-binding, antioxidant, and antibacterial properties of chitosans prepared from five different crab leg shell to abdomen shell percentage ratios (100:0, 75:25, 50:50, 25:75, and 0:100) were compared. The leg shell (LS) contained comparable protein (28.37% vs. 28.10%), ash (50.32% vs. 49.47%), and fat (0.26% vs. 0.27%), but lower chitin (19.80% vs. 21.92%) contents compared with the abdomen shell (AS). The physicochemical and functional properties of chitosans slightly differed depending on the LS:AS ratio used as a raw material. Increased AS ratio generally increased degree of deacetylation, dye-binding capacity and DPPH radical scavenging activity, but decreased viscosity, bulk density and antibacterial activity of chitosan. Nitrogen and ash contents of chitosan were not affected by LS: AS ratios of crab shell. All chitosans visually looked white. This study demonstrated that crab leg shell and abdomen shell, singularly or in combination, can be effectively utilized as a chitosan source.