세포외기질 단백질로 미세패턴화된 표면에서 성체 간엽 줄기세포의 형태변화와 세포점착차이에 관한 연구
분야
의약학 > 의공학
저자
김지연 ( Ji Youn Kim ) , 박용두 ( Yong Doo Park ) , 박정호 ( Jung Ho James Pak ) , 이규백 ( Kyu Back Lee ) , 선경 ( Kyung Sun )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2004년, 제1권 제2호, 157~163페이지(총7페이지)
파일형식
2a800004.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Patterning of surface using soft lithography is useful tools for regulating cellular morphology as well as function. Microcontact printing technology was used for patterning extracellular matrix (ECM) protein in this study. This pattern was used for controlling cellular adhesion as well as morphology of human mesenchymal stem cell. Polydimethylsiloxane (PDMS) molds was designed for controlling cellular adhesion by varying stripe width from 5 to 50 μm and fixing spacer to 20 μm. Glass surface was coated with extracellular matrix proteins such as fibronectin and vitronectin with different PDMS molds. Human mesenchymal stem cells were cultured on the patterned surfaces. Cells were aligned on the surfaces by changing cellular morphology. Actin filaments were aligned according to the protein stripe on the surfaces. The length of the actin fiber is longer as the stripe width is thinner. Proteins for focal contact such as vinculin, paxillin, and FAK were localized only on the ECM patterned surfaces. Especially, paxillin and FAK playing key role for focal adhesion were colocalized on the patterned surfaces. This study provides the controlling localization of focal adhesion proteins as well as morphology of human MSCs on the patterned surfaces. For further study, the effects of the localization of focal contacts proteins of MSCs to the stemness and differentiation will be investigated.
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