Original Article : A newly developed consensus polymerase chain reaction to detect Mycoplasma species using 16S ribosomal RNA gene
분야
농학 > 축산
저자
( Sunhwa Hong ) , ( Sang Ho Park ) , ( Yung Ho Chung ) , ( Okjin Kim )
발행기관
한국동물위생학회(구 한국가축위생학회)
간행물정보
한국가축위생학회지 (KOJVS) 2012년, 제35권 제4호, 289~294페이지(총6페이지)
파일형식
1v600832.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifi-cally to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma ge-nus- specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.
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