Breeding and Genetics : Gene Expression Profiling in Hepatic Tissue of two Pig Breeds
농학 > 축산
( Gul Won Jang ) , ( Kyung Tai Lee ) , ( Jong Eun Park ) , ( Hee Bal Kim ) , ( Tae Hun Kim ) , ( Bong Hwan Choi ) , ( Myung Jick Kim ) , ( Da Jeong Lim )
한국축산학회(구 한국동물자원과학회)
한국축산학회지 2012년, 제54권 제6호, 383~394페이지(총12페이지)
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    Microarray analyses provide information that can be used to enhance the efficiency of livestock production. For example, microarray profiling can potentially identify the biological processes responsible for the phenotypic characteristics of porcine liver. We performed transcriptome profiling to identify differentially expressed genes(DEGs) in liver of pigs from two breeds, the Korean native pigs(KNP) and Yorkshire pigs. We correctly identified expected DEGs using factor analysis for robust microarray summarization(FARMS) and robust multi-array average(RMA) strategies. We identified 366 DEGs in liver(p<0.05, fold- change>2). We also performed functional analyses, including gene ontology and molecular network analyses. In addition, we identified the regulatory relationship between DEGs and their transcription factors using in silico and qRT-PCR analysis. Our findings suggest that DEGs and their transcription factors may have a potential role in adipogenesis and/or lipid deposition in liver tissues of two pig breeds.
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