It has been widely recognized that bone marrow stromal cells (BMSCs) can be differentiated into osteoblasts, chondrocytes, and neuronal cells under the controlling of culture condition. In the present study, poly(L-lactide- co-glycolide) (PLGA) film was fabricated to release butylated hydroxyanisole (BHA) for the purpose of neurogenesis of BMSCs. PLGA films loaded BHA were manufactured as the concentrations of 200, 400, and 600 ? M/0.2 g PLGA and characterized by scanning electron microscope (SEM). The inverted microscope was used for the observation of the morphology of BMSCs cultivated on BHA/PLGA film during 5 days. MTT assay used for the measurement of the viability of the cells and cytotoxicity. After 5 days, differentiation of neuronal cells were confirmed by means of staining such as neurofilament (NF) and neuron-specific enolase (NSE). The changes of BMSCs to neuronal cell observed onto the fabricated PLGA film from after 1 day by the stimulation of BHA. The more the concentration of BHA increased, the less the viability of the cells, however, the cytotoxicity of the cells showed little effect until up to 600 ?M/0.2 g PLGA of concentration from the result of immunohistochemistry. It can be concluded that the controlled release of BHA from BHA loaded PLGA film might be differentiated BMSCs to neuronal cells due to the diffusion of BHA and biodegradation of PLGA. This study suggested that BHA/PLGA film is released the desired period in delivery system and useful neuronal growth culture of BMSCs for the application of neuronal regenerative medicine.