뷰틸레이트하이드록시애니솔이 서방화된 PLGA 필름을 이용한 골수간엽줄기세포의 신경세포화
분야
의약학 > 의공학
저자
이해방 ( Hai Bang Lee ) , 장우영 ( Woo Young Jang ) , 강길선 ( Gil Son Khang ) , 김선화 ( Seon Hwa Kim ) , 김문석 ( Moon Suk Kim ) , 이종문 ( John M. Rhee ) , 이일우 ( Il Woo Lee )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2005년, 제2권 제2호, 100~108페이지(총9페이지)
파일형식
2a800031.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    It has been widely recognized that bone marrow stromal cells (BMSCs) can be differentiated into osteoblasts, chondrocytes, and neuronal cells under the controlling of culture condition. In the present study, poly(L-lactide- co-glycolide) (PLGA) film was fabricated to release butylated hydroxyanisole (BHA) for the purpose of neurogenesis of BMSCs. PLGA films loaded BHA were manufactured as the concentrations of 200, 400, and 600 ? M/0.2 g PLGA and characterized by scanning electron microscope (SEM). The inverted microscope was used for the observation of the morphology of BMSCs cultivated on BHA/PLGA film during 5 days. MTT assay used for the measurement of the viability of the cells and cytotoxicity. After 5 days, differentiation of neuronal cells were confirmed by means of staining such as neurofilament (NF) and neuron-specific enolase (NSE). The changes of BMSCs to neuronal cell observed onto the fabricated PLGA film from after 1 day by the stimulation of BHA. The more the concentration of BHA increased, the less the viability of the cells, however, the cytotoxicity of the cells showed little effect until up to 600 ?M/0.2 g PLGA of concentration from the result of immunohistochemistry. It can be concluded that the controlled release of BHA from BHA loaded PLGA film might be differentiated BMSCs to neuronal cells due to the diffusion of BHA and biodegradation of PLGA. This study suggested that BHA/PLGA film is released the desired period in delivery system and useful neuronal growth culture of BMSCs for the application of neuronal regenerative medicine.
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