We regenerated a vascular media tissue by seeding smooth muscle cells on the inside of a hybrid vascular scaffold. The hybrid vascular scaffold consisted of two porous biodegradable poly(lactide-co-glycolide) (PLGA) thin layers on both the inner and the outer surfaces of the non-biodegradable expanded polytetrafluoroethylene (ePTFE) layer. The PLGA layers were designed to have porous structures for control of tissue regeneration by impregnating and then gas-foaming of ammonium bicarbonates as porogens of the biodegradable layers. To improve tissue regeneration, we coated gelatin polymers on inside the porous biodegradable PLGA surface. Media tissue regeneration was successfully achieved by replacing the PLGA layers and the tissues were observed by H&E staining and Sircol collagen assay. Tissue formation was dependent on cell culture period. While the two PLGA layers were expected to serve as biological layers of intima, media and adventitia of the blood vessel after their transformation into the engineered tissues, the non-biodegradable ePTFE in the middle of the hybrid scaffold did as a mechanically supporting layer.