PVA hydrogel, which prepared by freezing-thawing or irradiation crosslinking, is widely utilized in biomedical field as biocompatible hydrogels. In this study, we examined the gelation for PVA solution containing erythromycin( EM) as a model crystalline drug. EM loaded PVA solution was prepared in the presence of various additives such as ethyl carbitol, polyethylene glycol, glycerol, and Tween 80. No gelation of these solutions observed at room temperature. However, gelation due to crystallization was occurred by freezing-thawing method with dependence of additives. This result indicated that gelation of EM loaded PVA could be controlled by additives. To examine the formation of intermolecular hydrogen bonding between PVA and EM, EM loaded PVA solution with and without various additives was analyzed by using X-ray diffraction(XRD) and differential scanning calorimetry( DSC). The characteristic peak assignable to PVA or EM observed, but the peaks changed or disappeared in the presence of various additives. In addition, the crystallinity of EM loaded PVA solution decreased in the presence of various additives. In conclusion, we can control the gelation of EM loaded PVA solution through the addition of additives.