We have investigated differentiation of bone marrow stromal cells(BMSCs) through co-culture system with Schwann cells(SCs). Expression of the S-100, Schwann cell markers, was determined by immunocytochemical staining and reverse transcriptase polymer chain reaction(RT-PCR). The morphology and growth in terms of differentiated BMSC was observed by invert microscope. Following co-culture time with SCs, BMSCs underwent morphological changes similar to those of cultured SCs and stained positively for S-100 protein as well as high proliferation aspect were detected. Electrophoresis analysis revealed that the levels of S-100 on BMSC co-cultured with SCs related with other group maintained up to 21 days. These findings support the assertion that BMSCs can be differentiated into cells that are Schwann cell-like in terms of phenotype using Schwann cell only.