연구논문 : 인간 디스크세포와 PLGA/DBP 지지체를 이용한 생체조직공학적 바이오 디스크
분야
의약학 > 의공학
저자
고연경 ( Youn Kyung Ko ) , 김순희 ( Soon Hee Kim ) , 하현정 ( Hyun Jung Ha ) , 김문석 ( Moon Suk Kim ) , 한창환 ( Chang Whan Han ) , 손영숙 ( John M. Rhee ) , 이종문 ( Youngsuk Son ) , 이해방 ( Hai Bang Lee ) , 강길선 ( Gilson Khang )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2007년, 제4권 제1호, 67~72페이지(총6페이지)
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    영문초록
    We fabricated poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle( DBP)(PLGA/DBP) to investigate the effect of cell viability, proliferation and characteristic maintenance of intervertebral disc(IVD) cells. DBP-loaded PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in PLGA/DBP scaffold, and then cell viability and proliferation according to DBP content were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay. In MTT assay results, cell viability in scaffolds impregnated 20 and 80 wt% of DBP were higher than other scaffolds. Reverse transcript polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression of the disc target genes(aggrecan and type II collagen) of human IVD cells about various content of DBP. After implantation, thin sections were cut from paraffin embedded tissues and histological sections were stained H&E staining for observation of disc cell distribution. We concluded that the using of DBP(especially 20 wt% of DBP) in terms of scaffold fabrication for bio-disc with human IVD cells helps growth of disc cells maintained their phenotypes.
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