연구논문 : PLGA미립구의 크기에 따른 인간디스크세포의 증식 및 표현형 분석
분야
의약학 > 의공학
저자
소정원 ( Jeong Won So ) , 김순희 ( Soon Hee Kim ) , 백미옥 ( Mi Ock Baek ) , 임지예 ( Ji Ye Lim ) , 노혜원 ( Hye Won Roh ) , 이나리 ( Na Ri Lee ) , 김문석 ( Moon Suk Kim ) , 유규하 ( Gyu Ha Ryu ) , 조양하 ( Yang Ha Cho ) , 이승진 ( Seung Jin Lee ) , 민병현 ( Byoung Hyun Min ) , 강길선 ( Gil
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2007년, 제4권 제4호, 577~582페이지(총6페이지)
파일형식
2a800188.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    4,500원
    적립금
    135원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    영문초록
    The PLGA microspheres can create an environment conductive to promising candidate as an injectable system for tissue engineered intervertebral disc(IVD). The final goal of this study was to investigate the condition of PLGA microspheres as microcarriers for IVD cells. We prepared PLGA microspheres using oil-in-water(O/W) solvent evaporation method and investigated the size variation of the microspheres(180~425 μm). Human disc cells were used in a prospective study of attachment, proliferation, and gene expression within various size of PLGA microspheres. Mophology of cell attached on the microspheres was studied using scanning electron microscopy (SEM). Cellular behavior in response to various size of PLGA microspheres was analyzed using MTT assay and reverse transcriptase - polymerase chain reaction(RT-PCR). SEM showed that cells could be bridge between microspheres with elongated shapes. MTT assay revealed that the cells could be attach, proliferate and spread on PLGA microspheres, in particular on the ones having size below 250 μm. In RT-PCR results, we observed strongly specific gene expression of type collagen at more 250 μm microsphere. In conclusion, this result will be used to injectable system for disc regeneration.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일 help@reportshop.co.kr
    copyright (c) 2009 happynlife. steel All reserved.