연구논문 : PLGA 담체의 제조방법에 따른 골수간엽줄기세포의 골분화 유도
분야
의약학 > 의공학
저자
소정원 ( Jeong Won So ) , 김순희 ( Soon Hee Kim ) , 백미옥 ( Mi Ock Baek ) , 임지예 ( Ji Ye Lim ) , 노혜원 ( Hye Won Roh ) , 이나리 ( Na Ri Lee ) , 유규하 ( Gyu Ha Ryu ) , 조양하 ( Yang Ha Cho ) , 이승진 ( Seung Jin Lee ) , 강길선 ( Gilson Khang )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2008년, 제5권 제1호, 84~90페이지(총7페이지)
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2a800202.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    A key component in tissue engineering for bone regeneration is the scaffold that serves as a template for cell interaction and the formation of bone-extracellular matrix to provide structural support to the newly formed tissue. In this study, poly(L-Lactide-co-glicolide)(PLGA) scaffolds were prepared by solvent casting/salt-leaching, ice particle-leaching and gas-forming/salt-leaching. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole- 2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed ALP and osteocalcin m RNA expression. Scaffold with BMSCs were implanted into the back of athymic nude mouse to observe in vivo behavior. Thin section were cut from paraffin embedded tissues and histological sections were stained by trichrome, immunohistochemical, von kossa staining. The cell proliferation and ALP activity of BMSCs in the scafoolds was the highest in the scaffolds using solvent casting/salt-leaching method. In RT-PCR results, in the all type of scaffolds observed specific gene expression of ALP, osteocalcin. By histological staining, we could observed collagen and calcification region in scaffolds by solvent casting/salt leaching method. Thus Solvent casting/salt-leaching method provided highly porous structure with good interconnections between each pores, which can support the surface of cell proliferation, differentiation. This result indicates that solvent casting/salt-leaching is useful for osteogenesis of BMSCs.
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