PLGA 지지체에 함유된 DBP 함량이 줄기세포의 골분화에 끼치는 영향에 대한 in vitro 테스트
분야
의약학 > 의공학
저자
백미옥 ( Mi Ock Baek ) , 김순희 ( Soon Hee Kim ) , 소정원 ( Jung Won So ) , 임지예 ( Ji Ye Lim ) , 노혜원 ( Hye Won Roh ) , 이나리 ( Na Ri Lee ) , 유규하 ( Gyu Ha Ryu ) , 조양하 ( Yang Ha Cho ) , 이승진 ( Seung Jin Lee ) , 강길선 ( Gil Son Khang )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2008년, 제5권 제1호, 91~95페이지(총5페이지)
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2a800203.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Demineralized bone particles(DBP) exhibit osteoinductive properties and are very useful for bone formation and other orthopeadic applications. In this study, we investigated the osteogenic capabilities of rat bone marrow stromal cells(rBMSCs) in PLGA scaffold impregnated with various DBP contents. DBP-loaded PLGA scaffold (10, 20, 40, 80 % w/w) were prepared by solvent casting/salt leaching method. rBMSCs were seeded in PLGA and DBP/PLGA scaffolds, and then cellular viability and proliferation were assayed by water-soluble tetrazolium salt(WST-8) assay. Osteoblast differentiation of cells were analyzed by alkaline phosphatase(ALP) activity. Finally, reverse transcript polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression of the genes (ALP, osteocalcine and type I collagen) of osteogenic marker in rBMSCs depending on various content of DBP. These results could demonstrated that presence of DBP in PLGA might play important roles in osteoblast differentiation of rBMSCs.
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