Bone marrow stromal cells(BMSCs) can be differentiated into chondrocytes, osteocytes, neuronal cells under the controlling of culture condition. We fabricated butylated hydorxyanisole(BHA) loaded poly(L-lactide-coglycolide) (PLGA) scaffolds(BHA/PLGA) for neurogenesis of BMSCs and to observe the release behavior of BHA. BHA/PLGA scaffold were manufactured as the concentrations of 200, 400, and 600 μM of BHA by solvent casting/ salt leaching method. We isolated and cultured BMSCs from the tibias of adult female Fischer rat, we seeded BMSCs into the inner core of the hybrid scaffold. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H- tetrazolium bromide(MTT) test. Morphology of celluar adhesion were confirmed by scanning electron microscope SEM). The effect of BHA/PLGA scaffold on the neural differentiation of rat BMSCs were assessed RT-PCR. According to results, aspect of release of BHA in BHA/PLGA matrix showed that the initial burst of BHA was lower in scaffold than film and released continuously during the desired period in delivery system. BHA 600 μM loaded scaffold revealed low cell viability by MTT assay results. Mophology of BMSCs changed like a neural cell in 200 and 400 μM BHA/PLGA by SEM observation. In addition, 200 and 400 μM BHA/ PLGA scaffold showed higher expression of neural markers. We conclude that neural differentiation of BMSCs in BHA/PLGA scaffold could be possible for long period.