연구논문 : 3D 형태의 PLGA 지지체에서 뷰틸레이트하이드록시애니솔의 방출거동 및 골수간엽줄기세포의 신경세포화

분야: 의약학 > 의공학
저자: 오아영 ( A Young Oh ) , 김순희 ( Soon Hee Kim ) , 모종현 ( Jong Hyun Moh ) , 김근아 ( Geun Ah Kim ) , 이종문 ( John M. Rhee ) , 이해방 ( Hai Bang Lee ) , 강길선 ( Gil Son Khang )
발행기관: 한국조직공학·재생의학회
간행물정보: 조직공학과 재생의학 2008년, 제5권 제2호, 235~241페이지(총7페이지)
파일형식: 2a800220.pdf [무료 PDF 뷰어 다운로드]
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영문초록

Bone marrow stromal cells(BMSCs) can be differentiated into chondrocytes, osteocytes, neuronal cells under the controlling of culture condition. We fabricated butylated hydorxyanisole(BHA) loaded poly(L-lactide-coglycolide) (PLGA) scaffolds(BHA/PLGA) for neurogenesis of BMSCs and to observe the release behavior of BHA. BHA/PLGA scaffold were manufactured as the concentrations of 200, 400, and 600 μM of BHA by solvent casting/ salt leaching method. We isolated and cultured BMSCs from the tibias of adult female Fischer rat, we seeded BMSCs into the inner core of the hybrid scaffold. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H- tetrazolium bromide(MTT) test. Morphology of celluar adhesion were confirmed by scanning electron microscope SEM). The effect of BHA/PLGA scaffold on the neural differentiation of rat BMSCs were assessed RT-PCR. According to results, aspect of release of BHA in BHA/PLGA matrix showed that the initial burst of BHA was lower in scaffold than film and released continuously during the desired period in delivery system. BHA 600 μM loaded scaffold revealed low cell viability by MTT assay results. Mophology of BMSCs changed like a neural cell in 200 and 400 μM BHA/PLGA by SEM observation. In addition, 200 and 400 μM BHA/ PLGA scaffold showed higher expression of neural markers. We conclude that neural differentiation of BMSCs in BHA/PLGA scaffold could be possible for long period.

#Bone marrow stromal cells # PLGA # BHA # neurogenesis

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