Original Paper : Chondrogenesis of Rat Bone Marrow Stromal Cells in Transforming Growth Factor-β1 Loaded Alginate Bead in In Vivo

분야: 의약학 > 의공학
저자: ( Soon Hee Kim ) , ( Ji Wook Jang ) , ( Su Hyun Jung ) , ( Jin Hee Choi ) , ( Hyun Jung Ha ) , ( John M. Rhee ) , ( Young Sun Kang ) , ( Gilson Khang )
발행기관: 한국조직공학·재생의학회
간행물정보: 조직공학과 재생의학 2008년, 제5권 제4호, 650~659페이지(총10페이지)
파일형식: 2a800262.pdf [무료 PDF 뷰어 다운로드]
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영문초록

This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H&E, Alcian Blue, Safranin-O and immunohistochemistry( IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.

#Chondrogenesis # BMSC # alginate bead # TGF- β1 # in vivo

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