5-Fluorouracil(5-FU) has been widely used to treat cancer as types of iv and oral adminstration. However, it induces many side effects and has short half-life time of 10-20 minutes. Furthermore, it has low bioavailability of 20 % due to the hepatic first pass effect. Therefore, we developed novel TDDS using hydrogel and microneedle system. 5, 8 and 10% w/v of Carbopol 940NF was added in hydrogel containing 5-FU to improve permeability. Viscosity of hydrogel increased with increase of Carbopol contents. We applied these novel DDS to hairless mice skin treated and untreated with microneedle system and evaluated drug permeability into skin. The hydrogel containing 8% w/v Carbopol showed best permeability among three groups. We concluded viscosity of drug acts important factor in permeability into skin and bioavailability could be improved through our novel TDDS.