Various gene transfer play important roles in the development of the controlled release of nucleic acid. In this review, we describe a relatively new approach to the immobilization and release of DNA from surfaces and detailed overview of the state of the art. First, methods based on the layer-by-layer assembly of thin multilayered films will be discussed. The applications of layer-by-layer assembly to the fabrication of DNA-containing surfaces will be then presented. We believe this approach can contribute potential method for spatial and/or temporal control over the delivery of DNA.