알지네이트 스펀지 세포지지체에서 인체 지방조직 추출 줄기세포의 연골분화
분야
의약학 > 의공학
저자
최문섭 ( Moon Seop Choi ) , 전영준 ( Young Joon Jun ) , 조현미 ( Hyun Mi Cho ) , 오득영 ( Deuk Young Oh ) , 안상태 ( Sang Tae Ahn ) , 한동근 ( Dong Keun Han ) , 이종원 ( Jong Won Rhie )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2008년, 제5권 제4호, 842~848페이지(총7페이지)
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    영문초록
    Recently it has been proven that population of stem cells obtained from stromal vascular fraction of fat tissue have the multilineage potential which is a capability of differentiation into bone, cartilage, fat, and muscle. In addition, a surgeon can easily harvest human adipose tissue using by minimal invasive technique such as a liposuction. In this article, I hypothesized that human adipose tissue contains the stem cells and has an capability of chondrogenic differentiation in alginate sponge scaffolds. Alginate sponge scaffolds were made by lyophilizing of alginate throughout alginate gel state and human adipose tissue was obtained from a liposuction. In experimental group, human adipose-derived stem cells in alginate sponge scaffolds, were differentiated with chondrogenic medium consisted of transforming growth factor-β1 and dexamethasone. Control group was defined as a culture with complete medium. After differentiation at 1, 2, and 3 weeks, gene expression was examined by reverse transcriptase polymerase chain reaction(RT-PCR) for collagen type II and link. In addition, content of glycosaminoglycan and Alcian blue stain were carried out for confirming of chondrogenesis. RT-PCR results show gene encodings of collagen type II and link in experimental group. The content of glycosaminoglycan in experimental group was greater than that in control group. In Alcian blue stain, sulfated proteoglycan was stained with blue color in experimental group. Consequently it was proven that adipose-derived stem cells have a capability of chondrogenic differentiation in alginate sponge scaffolds. In a view point of cell therapy, tissue engineering of adipose-derived stem cells is able to apply for reconstruction of tissue defects, congenital, and esthetics.
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