DBP스폰지에서 BMP-2의 효과확인 및 섬유륜 조직재생
분야
의약학 > 의공학
저자
최진희 ( Jin Hee Choi ) , 장지욱 ( Ji Wook Jang ) , 김순희 ( Soon Hee Kim ) , 홍희경 ( Hee Kyung Hong ) , 민병현 ( Byung Hyun Min ) , 손영숙 ( Youngsook Son ) , 이종문 ( John M Rhee ) , 강길선 ( Gilson Khang )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2008년, 제5권 제4호, 877~884페이지(총8페이지)
파일형식
2a800290.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used as biomaterials. In this study, we evaluated 3-dimensional DBP sponge and Collagen sponge on proliferation and phenotype maintenance of annulus fibrosus cells. DBP sponge were prepared by freeze-drying method after addition 2 wt% DBP solution. sponge was crosslinked with 1-ethyl-(3-3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution with 50 mM concentration for 24 hrs and lyophilized. We seeded cells in DBP sponge and Collagen sponge. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. DBP sponge and Collagen sponge were characterized by scanning electron microscopy(SEM). Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression for type I collagen, type II collagen, aggrecan and osteonectin and glycosaminoglycan(GAG) quantity from annulus fibrosus cells in sponges. In MTT assay result, DBP sponges were higher cell viability. In SEM observation, we observed that DBP sponge has uniform porosity. In addition, annulus fibrosus stronly expressed their specific mRNA. and produced well sGAG in DBP sponge. This result indicates that DBP sponge is useful for intervertebral disc regeneration.
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