Resveratrol is a polyphenol found in food sources such as peanuts, berries, grapes and red wine. It has been touted for, among other actions, beneficial effects on the cardiovascular system. Consequently, it has been marketed as a nutritional supplement for healthy individuals. Using a double-blind, crossover design, we tested the hypothesis that chronic supplementation with resveratrol attenuates blood pressure at rest and during skeletal muscle contraction and augments endothelial function and exercise-induced increases in skeletal muscle blood flow in this group.Mean arterial blood pressure (MAP), heart rate (HR), endothelial function (evaluated via flow mediated dilation) brachial artery diameter (ultrasound) and rhythmic forearm contraction-induced increases in skeletal muscle blood flow (Doppler/ultrasound) were assessed before and after chronic (500 mg/day for 28-32 days, n = 14) supplementation with resveratrol. Compared to placebo, resveratrol supplementation had modest effects on resting MAP(82 ± 2 vs. 80 ± 2 mmHg) (P < 0.05). However, no effects of this polyphenol were seen on HR, flow mediated dilation or exercise-induced increases in MAP, brachial artery diameter or blood flow. We concluded that 30 days of supplementation with a commercially available dose of resveratrol (500 mg/day) have minimal, if any, beneficial cardiovascular effects in healthy individuals and do not improve endothelial or cardiovascular function during exercise.