Microarray 분석을 이용한 유채 종자성숙단계별 유전자 발현 양상
농학 > 농화학
노경희 ( Kyung Hee Roh ) , 박종석 ( Jong Sug Park ) , 김종범 ( Jong Bum Kim ) , 김현욱 ( Hyun Uk Kim ) , 이경렬 ( Kyeong Ryeol Lee ) , 김순희 ( Sun Hee Kim )
한국응용생명화학회(구 한국농화학회)
Journal of Applied Biological Chemistry 2012년, 제55권 제4호, 227~234페이지(총8페이지)
06005965.pdf [무료 PDF 뷰어 다운로드]
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    We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiationrelated genes were highly expressed at 10 DAF, whereas energyrelated genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.
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