영문초록
We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiationrelated genes were highly expressed at 10 DAF, whereas energyrelated genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.
추천자료
[유전자조합식품] 유전자조합식품에 관한 환경이슈
유전자 조작 농산물
[생명공학] 생명공학의 정의, 발전양상, 시장규모와 선진국의 생명공학 동향, 생명공학의 기술이용, 생명공학의 찬성, 반대의견 및 생명공학 육성정책 추진 방향 분석
응용생물학 기초실험 - 종자발아 실험
[인간복제]인간복제의 정의,배경, 인간복제의 영향,대가, 인간복제 관련 법률, 인간복제의 동향, 향후 인간복제의 과제 분석
[인간배아복제]인간배아복제의 의미,필요성, 배아줄기세포공학기술, 인간배아복제의 문제점, 인간배아복제의 반대론,찬성론,시사점
[환경문제] 유전자조합식품
[생명공학] 줄기세포의 정의와 줄기세포연구의 필요성, 문제점, 찬반론 및 줄기세포를 이용한 치료전략 그리고 줄기세포 치료기술의 수요, 전망 분석
[줄기세포] 줄기세포의 정의와 줄기세포연구의 필요성, 문제점, 찬반론 및 줄기세포를 이용한 치료전략 그리고 줄기세포 치료기술의 수요, 전망 분석
[환경과학] 식량문제