원저 : Human Gingival Fibroblasts의 분화와 증식에서 CGFs의 생물학적 효과
분야
의약학 > 기초치의학
저자
박성일 ( Sung Il Park ) , 배현숙 ( Hyun Sook Bae ) , 홍기석 ( Ki Seok Hong )
발행기관
한국치위생과학회
간행물정보
치위생과학회지 2012년, 제12권 제6호, 689~695페이지(총7페이지)
파일형식
1g100693.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    The aim of this study was to elucidate the effects of concentrated growth factors (CGFs) on human gingival fibroblasts in vitro. Blood was collected from three male volunteers (average age 27 years). CGFs were prepared using standard protocols. The CGF exudates were collected at the following culture time points: 1, 7, 14, and 21 days. The levels of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in CGFs were quantified. The CGF exudates were then used to culture human gingival fibroblasts. The biologic characteristics of these fibroblasts were analyzed in vitro for 21 days. Platelet-rich plasma released the highest amounts of TGF-β1 and PDGF-BB on the first day. The level of TGF-β1 had decreased slightly by day 7, although the difference compared to levels at day 1 was not statistically significant. However, by days 14 and 21, levels of TGF-β1 had dropped significantly compared to day 1 levels. The levels of PDGF-BB at days 7, 14, and 21 did not differ significantly from that measured on day 1. CGFs maintained the release of autologous growth factors for a reasonable period of time (7 days for TGF-β1 and 21 days for PDGF-BB). Gingival fibroblasts treated with CGF exudates collected at day 14 reached peak viability and synthesized type I collagen. Furthermore, the CGF exudates exerted positive effects on the proliferation and differentiation of these cells at days 1, 7, 14, and 21. The findings of this study suggest that treatment with CGFs represents a promising method of enhancing mucosal healing following surgical procedures.
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