케라틴/PLGA 지지체가 골수간엽줄기세포의 신경분화에 미치는 영향
분야
의약학 > 의공학
저자
오아영 ( A Young Oh ) , 김순희 ( Soon Hee Kim ) , 김초민 ( Cho Min Kim ) , 정수현 ( Su Hyun Jung ) , 전나리 ( Na Ri Jeon ) , 홍현혜 ( Hyun Hye Hong ) , ( Sang Jin Lee ) , ( Mark Van Dyke ) , ( James J Yoo ) , 이동원 ( Dong Won Lee ) , 이종문 ( John M Rhee ) , 강길선 ( Gilson Khang
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제1호, 112~118페이지(총7페이지)
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2a800319.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Bone marrow stromal cells(BMSCs) exhibit multiple traits of a stem cell population, and they can expand many times in vitro and be induced to differentiate into multiple cell types. Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. We think keratins are useful as natural biomaterial. We examined the effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs. We developed the keratin loaded poly(L-lactide-co-glycolide)(PLGA) scaffolds. Keratin/PLGA 0, 20 and 50 wt% scaffolds were prepared by solvent casting/salt leaching method. BMSCs were harvested from the femurs of adult female Fischer rat. These cells were seeded in prepared Keratin/PLGA scaffold and cultured in Medium 50 ml DMEM, 2 %DMSO, 200 ?M BHA, 25 ?M valproic acid, 10 ?M forskolin, 1 ?M hydro-cotisone, 5?g/ml insulin for 5 day. The effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs were assessed in culture using the MTT assay and RT-PCR was conducted to confirm mRNA expression of NSE and Nf for neural marker. We confirmed that effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs. According to our results, 20 wt% keratin/ PLGA scaffold have good cell compatibility and the expression of neural markers was higher. In conclusion, Keratin/ PLGA scaffold, on which neural differentiation of rat BMSCs was possible.
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