Original Paper : Newborn Calf Serum Retards Loss of the Chondrocytic Phenotype during In Vitro Cell Expansion

분야: 의약학 > 의공학
저자: ( Jung Sun Lee ) , ( Jin Yeon Lee ) , ( Eun Kyung Lee ) , ( Young Sook Son )
발행기관: 한국조직공학·재생의학회
간행물정보: 조직공학과 재생의학 2009년, 제6권 제1호, 229~235페이지(총7페이지)
파일형식: 2a800333.pdf [무료 PDF 뷰어 다운로드]
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연관 논문

논문 ; 육종 번식 : 소 난포란의 체외성숙중 단백질 합성에 관한 연구 : 부착난구세포에서의 경시적 변화 ( Articles ; Breeding and Reproduction : Time - Course Biosynthesis of Polypeptides in Cumulus Cells during Bovine Oocyte Maturation In Vitro )

TGF-beta Stimulates Conversion of Fibrocartilage Phenotype of Costal Chondrocytes

배양된 인체 백혈구 세포의 노화 진행에 따른 SCE 빈도의 변화 양상에 관하여 ( The Effect of Aging on Spontaneous and Mutagen Induced SCE Frequencies in Human Lymphoid Cells Cultivated with three Different Kinds of Serum )

신생아제대혈청이 배양중인 생쥐 난자-난구 복합체의 홀몬생성에 미치는 영향 ( Effect of Human Cord Serum on the Hormone Production by Mouse Oocyte Cumulus Complexes in vitro )

Original Article : Cartilage Tissue Engineering using Chondrocyte-Derived Extracellular Matrix Scaffold Suppressed Vessel Invasion During Chondrogenesis of Mesenchymal Stem Cells In Vivo

영문초록

Chondrocytes isolated from cartilage tissue lose their chondrocytic phenotype during in vitro cell expansion, an event which has severely limited the advancement of tissue engineered cartilage and chondrocyte cell therapy. In an attempt to retard this loss of phenotype, we cultured costal chondrocytes in a supplementation of newborn calf serum(NCS) as opposed to the more commonly used fetal bovine serum(FBS). The costal chondrocytes cultured in the NCS medium maintained their chondrocytic phenotype longer than those cultured in the FBS medium, resulting in approximately five fold more cells expressing type II collagen and maintaining a round chondrocytic morphology after 3 weeks in culture. To evaluate their redifferentiation, the expanded chondrocytes were then seeded on a chitosan scaffold and cultured under NCS or FBS conditions. The differential effect of NCS on the maintenance of chondrocytic phenotype was also manifest in the high density three-dimensional culture, as evident by higher sustained expression of glucosaminoglycan than that seen in FBS medium. These results suggest that either FBS may act to stimulate the fibroblastic conversion of chondrocytes or that NCS may serve to retard it.

#Costal chondrocyte # fibroblastic transition # NCS # tissue engineered cartilage

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