중간엽 줄기세포의 연골세포로의 분화능에 (-)-에피갈로카테킨 갈레이트가 미치는 영향
분야
의약학 > 의공학
저자
송지혜 ( Ji Hae Song ) , 박부영 ( Bu Young Park ) , 김종일 ( Jong Il Kim ) , 최성훈 ( Sung Hun Choi ) , 김동일 ( Dong Il Kim )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제1호, 243~249페이지(총7페이지)
파일형식
2a800334.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    The cell therapy using tissue-engineered cartilage is essential because the surgical operation for the repair of defected cartilage is not sufficient. Mesenchymal stem cells(MSCs) are an ideal candidate for use in cell-based therapy because of their self-renewal ability and differentiation capability into specific cells such as chondrocytes. Especially, human umbilical cord blood(UCB) is an attractive cell source because it can be obtained easily and the process is noninvasive and painless. However, the life span of MSCs are limited and the accumulation of intracellular oxidative damage by aging causes the loss of differentiation capacity during ex vivo expansion. (-)-Epigallocatechin gallate(EGCG) is known as an anti-oxidant agent which has a role as a free radical scavenger. It can improve long-term cell viability. Therefore, differentiation capacity can be maintained. In this study, effects of EGCG on chondrogenic potential of UCB-MSCs were investigated. The proliferation and differentiation capacity of UCBMSCs was characterized during expansion. Optimal concentration of EGCG was decided. Addition of EGCG in the media increased the cell viability during chondrogenic induction. Glycosaminoglycan(GAG), a cartilage-specific extracellular matrix(ECM), was also increased. It was confirmed that EGCG could improve the chondrogenic potential of UCB-MSCs by reducing oxidative stress. These results can contribute to the repair of damaged articular cartilage using UCB-MSCs.
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