Calcium sulfate is widely used for bone grafts as binder and expander. It was also reported that carboxymethylcellulose(CMC) can improve chemical characteristics for clinical applications when it is used as binder of calcium sulfate and demineralized bone matrix(DBM) for particulate bone grafts. DBM is an osteoinductive material which has been used to reconstruct bone tissue in maxillocraniofacial surgery, periodontics, and orthopedics. In this study, the effects of CMC-based putties containing DBM and/or calcium sulfate hemihydrate were evaluated in rat skull defects. Radiographs and histological evaluation of defect area after a period of time showed healing progress and new bone appeared greater for the treatment of CMC-based calcium sulfate hemihydrate putty compared to the untreated defect. When DBM was added to the putty, the most prominent bone regeneration effect was observed. We conclude that fast-resorbing CMC-based calcium sulfate hemihydrate putty containing DBM is effective for healing from the defective bone. CMC-based calcium sulfate may be used as a carrier and binder of bone graft materials for the restoration of bony defects.