인간 지방조직유래 줄기세포의 PLCL/피브린 복합지지체에서 연골세포로의 분화
분야
의약학 > 의공학
저자
김상희 ( Sang Hee Kim ) , 정영미 ( Young Mee Jung ) , 김상헌 ( Sang Heon Kim ) , 이종원 ( Jong Won Rhie ) , 김영하 ( Young Ha Kim ) , 김철근 ( Chul Geun Kim ) , 김수현 ( Soo Hyun Kim )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제1호, 313~319페이지(총7페이지)
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2a800342.pdf [무료 PDF 뷰어 다운로드]
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    In cartilage tissue engineering, choosing on an appropriate cell source and a scaffold for cartilaginous tissue formation seriously influences the clinical application of an engineered cartilage. The purpose of this study is to investigate chondrogenic differentiation of human adipose tissue-derived stromal cells(hASCs) by hybridizing of fibrin gels for mimicking 3-dimensional environments of native cartilage and a poly(L-lactide-co-ε-caprolactone)(PLCL) scaffold. PLCL scaffolds with 85% porosity and 300-500 ?m pore size were fabricated by a gel-pressing method. For examining chondrogenic differentiation of hASCs, cells were mixed with 0.5% fibrin solutions and subsequently they were seeded onto PLCL scaffolds. After that, cell-scaffold constructs were implanted subcutaneously in nude mice for up to 8 weeks. Specimens were harvested after 8 weeks and analyzed. From in vivo studies, the constructs used with fibrin-PLCL hybrid scaffolds showed more depositions of cartilage-specific ECM components, such as GAGs and collagen type II, as compared to the constructs used with PLCL scaffolds alone. Furthermore, the constructs seeded with hASCs that were induced to chondrogenesis in vitro prior to implantation improved cartilage matrix deposition as compare to the constructs seeded with non-induced hASCs. These results indicated that the use of the fibrin gels-PLCL hybrid scaffold could enhance chondrogenic differentiation of hASCs and cartilaginous tissue formation. Also, the induction for chondrogenesis of hASCs prior to implantation in vitro could affect cartilaginous tissue formation in vivo. In conclusion, the hybrid system which was fabricated with the inoculation of ASCs and fibrin gels onto a PLCL scaffold could be a meaningful system in cartilage tissue engineering.
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