We tried to develop a simple method to fabricate fibrin-based dermal equivalent and determine the effect of culture condition on cell growth and gene expression. Human dermal fibroblasts(DFs) were encapsulated within fibrin and fabricated DFs-populated fibrin lattice(FPFL). In Group I, DFs-populated fibrin lattice(FPFL) was detached from culture plate immediately after polymerization and cultured in free floating(FF) condition for 8 days. In Group II, FPFL was cultured in attached condition for 3 days and then FF condition for 5 days. In Group III, FPFL was cultured in attached condition for 7 days and then cultured in FF condition for 1 day. The cell growth and gene expression were significantly higher in Group III than those of Group I and Group II(p<0.01), but lattice contraction was lowest. We used attached-delayed-released process to fabricate self assembled dermal equivalent. First, FPFL was cultured in attached condition for 7 days and detached in peripheral margin which resulted in contraction and spontaneous release of FPFL. The released FPFL cultured for 1 day for compaction, organization, and self assembly of lattices. We could successfully fabricate a self-assembled dermal equivalent based showing stretchable mechanical property, incorporation of angiogenic factors and newly synthesized extracellular matrices.