Original Article : Development of a Perfusion Flow Bioreactor System for Culturing Human Alveolar Bone Marrow Stem Cells
분야
의약학 > 의공학
저자
( Ki Taek Lim ) , ( Chong Su Cho ) , ( Yun Hoon Choung ) , ( Hoon Seon Woo ) , ( Hyun Mok Son ) , ( Soo Jung Baik ) , ( Jang Ho Kim ) , ( Sung Min Hong ) , ( Joo Young Park ) , ( Soung Hoo Jeon ) , ( Pill Hoon Choung ) , ( Jong Hoon Chung )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제14호, 1410~1419페이지(총10페이지)
파일형식
2a800457.pdf [무료 PDF 뷰어 다운로드]
  • ※ 본 자료는 참고용 논문으로 수정 및 텍스트 복사가 되지 않습니다.
  • 구매가격
    4,500원
    적립금
    135원 (구매자료 3% 적립)
    이메일 발송  스크랩 하기
    자료 다운로드  네이버 로그인
    영문초록
    We have developed and evaluated a novel perfusion flow bioreactor system which facilitates the observation of living cells for extended periods of time. Cell viability showed that cell growth rate cultured (without medium perfusion) using the novel flow perfusion chamber system revealed a similar trend (p>0.05) compared to that of static culture. Also, the cell viability under the static culture (without perfusion) and flow perfusion culture (flow rate: 0.03 ml/min) with the developed perfusion bioreactor system was compared. The cell growth rate of the flow perfusion culture was significantly higher (p<0.05) than that of static culture for 4 days. The cell growth rate was efficiently increased to about 10-15% with the precision perfusion culture. These results indicate that a bioreactor system that can provide mechanical signals has important applications. Additionally, the developed cell culture chamber system has been successfully used to maintain and record the morphology of cultured alveolar bone marrow stem cells. It was possible to monitor and capture the live cell morphology cultured in the perfusion bioreactor. The characteristics of the bioreactor developed in this study was fast to culture stem cells with mechanical stimulation and to monitor live cell imaging while promoting healthy cellular activity outside of an incubator environment. Through experimental results, the developed perfusion bioreactor could increase cell growth with proper flow based on mechanical stimuli.
    사업자등록번호 220-87-87785 대표.신현웅 주소.서울시 서초구 방배로10길 18, 402호 대표전화.070-8809-9397
    개인정보책임자.박정아 통신판매업신고번호 제2017-서울서초-1765호 이메일 help@reportshop.co.kr
    copyright (c) 2009 happynlife. steel All reserved.