Bone marrow derived mesenchymal stem cell(BMSC) based cell therapy can promote functional recovery and regeneration in central nervous system(CNS) disease models. These beneficial effects have been primarily attributed to the differentiation potential into varieties of cell lineages and the therapeutic paracrine factors secreted by BMSC after transplantation. Many secreted factors from BMSC have been identified recently in the conditioned media. However, the effects of these factors on the neuronal regeneration need further verification with relevant neuronal models. Ntera-2 neurons have been used for the therapy of several different CNS diseases in animal models. Recently some clinical trials have confirmed the safety and potential efficacy of Ntera-2 neurons for stroke therapy. In this study, the Ntera-2 neuron model was used to directly verify the effect of BMSC conditioned media(BMSCCM) on neurite outgrowth in vitro. Our study confirmed that the BMSC-CM could increase both the neurite length and branch number of Ntera-2 neurons, compared to the control group with non-conditioning media. Since the BMSC can secret many therapeutic factors into the conditioned media, our data support the theory that BMSC promote neurite outgrowth through secreting paracrine factors.