연골 특이적 ECM 및 영양세포가 골막 유래 전구세포의 생존도와 GAG 생성량에 미치는 영향
분야
의약학 > 의공학
저자
김종일 ( Jong Il Kim ) , 박부영 ( Bu Young Park ) , 임동균 ( Dong Kyoon Lim ) , 최성훈 ( Sung Hun Choi ) , 김동일 ( Dong Il Kim )
발행기관
한국조직공학·재생의학회
간행물정보
조직공학과 재생의학 2009년, 제6권 제4호, 647~652페이지(총6페이지)
파일형식
2a800385.pdf [무료 PDF 뷰어 다운로드]
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    영문초록
    Periosteum-derived progenitor cells(PDPCs) have a capacity to differentiate into a number of connective tissue lineage. They are an attractive source of chondrocyte-progenitor cells. When these cells are cultured in a threedimensional format in the presence of transforming growth factor-b3(TGF-b3), they showed characteristic morphological changes with the deposition of cartilaginous extracellular matrix(ECM). In this study, effects of various cartilage-specific ECMs or feeder cells on the chondrogenesis of PDPCs were investigated. Application of this system resulted in a more functional format and enhanced the cell viability than the chondrogenesis without cartilage-specific ECMs or feeder cells. Differentiation was found to be dependent on immunophenotypes and was interrelated with passage number. FACS analysis on CD29, CD44, CD105 by serial passage numbers of PDPCs provided an optimal seeding time for chondrogenesis. Addition of chondroitin 6-sulfate, known as cartilage-specific ECM, in induction media enhanced chondrogenesis, as indicated by the synthesis of glycosaminoglycan(GAG). When chondrocytes were used as feeder cells, the cell viability was increased during chondrogenesis.
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