Because osteoporosis is known to influence the late period of bone healing, bone formation around titanium implant is deteriorated in osteoporotic patient. The purpose of this study was to investigate the effect of calcium and vitamin D diet on the expressions of TGF-β1, decorin and biglycan in the peri-implant bone of induced osteoporotic rats. After 40 rats, 10 weeks of age, were ovarietomized, 20 rats were supplemented with calcium and vitamin D as a experimental group and 20 rats were without calcium and vitamin D as a control group. Titanium screw implant(Cowellmedi, KOREA, diameter, 2.0 mm; length, 3.5 mm; pitch-height 0.4 mm) were planted into tibia of 40 rats. The rats were sacrificed at different time interval(1, 2, and 4 weeks after implantation) and then immunohistochemistry for the expression of TGF-β1, decorin and biglycan was performed. The expression level of TGF-β1 and decorin in experimental groups was much higher than the control group at 1 week. The expression of biglycan in control group was observed at 1 and 2 weeks and this expression level was higher than that of experimental group. Considering calcium and vitamin D diet increased the expression of TGF-β1 and decorin in osteoporotic rats, we suggest that those diet might help early bone formation around titanium implant in osteoporotic patients.